Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RBBP4

Cell type

Cell type Class
Muscle
Cell type
Rh-5
NA
NA

Attributes by original data submitter

Sample

source_name
RH5
cell line
RH5
chip antibody
RBBP4, Epigentek (catA-2703 lotA511004 pAb)
enrichment target
RBBP4
condition
NuRD Complex
target function
no treatment

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
harvesting protocol: ChIP samples harvested after fixation, sonicated, processed and ChIP-enriched via standard protocols (iDeal ChIP-seq kit for Transcription Factors, Diagenode). DNA was extracted from ChIP-seq samples prepared following the iDeal ChIP-seq kit for Transcription Factors (Diagenode) protocol. Standard Illumina barcodes were introduced during library preparation with the Illumina TruSeq ChIP Sample Prep Kit 48 Samples - Set A Cat# IP-202-1012

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
37290213
Reads aligned (%)
95.3
Duplicates removed (%)
56.7
Number of peaks
1770 (qval < 1E-05)

hg19

Number of total reads
37290213
Reads aligned (%)
94.7
Duplicates removed (%)
57.4
Number of peaks
1773 (qval < 1E-05)

Base call quality data from DBCLS SRA